The nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha.
Identifieur interne : 002F87 ( Main/Exploration ); précédent : 002F86; suivant : 002F88The nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha.
Auteurs : Bing Zhou [République populaire de Chine] ; Junli Liu ; Qiuna Wang ; Xuan Liu ; Xiaorong Li ; Ping Li ; Qingjun Ma ; Cheng CaoSource :
- Journal of virology [ 1098-5514 ] ; 2008.
Descripteurs français
- KwdFr :
- Actines (métabolisme), Biosynthèse des protéines (physiologie), Cartographie d'interactions entre protéines, Cytocinèse (physiologie), Facteur-1 d'élongation de la chaîne peptidique (métabolisme), Humains, Immunoprécipitation, Liaison aux protéines, Lignée cellulaire, Lymphocytes (virologie), Prolifération cellulaire, Protéines nucléocapside (métabolisme), Résonance plasmonique de surface, Technique de Far-Western, Techniques de double hybride, Virus du SRAS (physiologie).
- MESH :
- métabolisme : Actines, Facteur-1 d'élongation de la chaîne peptidique, Protéines nucléocapside.
- physiologie : Biosynthèse des protéines, Cytocinèse, Virus du SRAS.
- virologie : Lymphocytes.
- Cartographie d'interactions entre protéines, Humains, Immunoprécipitation, Liaison aux protéines, Lignée cellulaire, Prolifération cellulaire, Résonance plasmonique de surface, Technique de Far-Western, Techniques de double hybride.
English descriptors
- KwdEn :
- Actins (metabolism), Blotting, Far-Western, Cell Line, Cell Proliferation, Cytokinesis (physiology), Humans, Immunoprecipitation, Lymphocytes (virology), Nucleocapsid Proteins (metabolism), Peptide Elongation Factor 1 (metabolism), Protein Binding, Protein Biosynthesis (physiology), Protein Interaction Mapping, SARS Virus (physiology), Surface Plasmon Resonance, Two-Hybrid System Techniques.
- MESH :
- chemical , metabolism : Actins, Nucleocapsid Proteins, Peptide Elongation Factor 1.
- physiology : Cytokinesis, Protein Biosynthesis, SARS Virus.
- virology : Lymphocytes.
- Blotting, Far-Western, Cell Line, Cell Proliferation, Humans, Immunoprecipitation, Protein Binding, Protein Interaction Mapping, Surface Plasmon Resonance, Two-Hybrid System Techniques.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1alpha), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin (F-actin). In vitro and in vivo interaction was then confirmed by immuno-coprecipitation, far-Western blotting, and surface plasmon resonance. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1alpha, inhibiting protein translation and cytokinesis by blocking F-actin bundling. Proliferation of human peripheral blood lymphocytes and other human cell lines was significantly inhibited by the infection of recombinant retrovirus expressing SARS-CoV N protein.
DOI: 10.1128/JVI.00133-08
PubMed: 18448518
Affiliations:
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Le document en format XML
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first="Qiuna" last="Wang">Qiuna Wang</name></author><author><name sortKey="Liu, Xuan" sort="Liu, Xuan" uniqKey="Liu X" first="Xuan" last="Liu">Xuan Liu</name></author><author><name sortKey="Li, Xiaorong" sort="Li, Xiaorong" uniqKey="Li X" first="Xiaorong" last="Li">Xiaorong Li</name></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18448518</idno><idno type="pmid">18448518</idno><idno type="doi">10.1128/JVI.00133-08</idno><idno type="wicri:Area/PubMed/Corpus">001B57</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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Zhou</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Junli" sort="Liu, Junli" uniqKey="Liu J" first="Junli" last="Liu">Junli Liu</name></author><author><name sortKey="Wang, Qiuna" sort="Wang, Qiuna" uniqKey="Wang Q" first="Qiuna" last="Wang">Qiuna Wang</name></author><author><name sortKey="Liu, Xuan" sort="Liu, Xuan" uniqKey="Liu X" first="Xuan" last="Liu">Xuan Liu</name></author><author><name sortKey="Li, Xiaorong" sort="Li, Xiaorong" uniqKey="Li X" first="Xiaorong" last="Li">Xiaorong Li</name></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins (metabolism)</term><term>Blotting, Far-Western</term><term>Cell Line</term><term>Cell Proliferation</term><term>Cytokinesis (physiology)</term><term>Humans</term><term>Immunoprecipitation</term><term>Lymphocytes (virology)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Peptide Elongation Factor 1 (metabolism)</term><term>Protein Binding</term><term>Protein Biosynthesis (physiology)</term><term>Protein Interaction Mapping</term><term>SARS Virus (physiology)</term><term>Surface Plasmon Resonance</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Actines (métabolisme)</term><term>Biosynthèse des protéines (physiologie)</term><term>Cartographie d'interactions entre protéines</term><term>Cytocinèse (physiologie)</term><term>Facteur-1 d'élongation de la chaîne peptidique (métabolisme)</term><term>Humains</term><term>Immunoprécipitation</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Lymphocytes (virologie)</term><term>Prolifération cellulaire</term><term>Protéines nucléocapside (métabolisme)</term><term>Résonance plasmonique de surface</term><term>Technique de Far-Western</term><term>Techniques de double hybride</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Actins</term><term>Nucleocapsid Proteins</term><term>Peptide Elongation Factor 1</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Actines</term><term>Facteur-1 d'élongation de la chaîne peptidique</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Biosynthèse des protéines</term><term>Cytocinèse</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cytokinesis</term><term>Protein Biosynthesis</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Far-Western</term><term>Cell Line</term><term>Cell Proliferation</term><term>Humans</term><term>Immunoprecipitation</term><term>Protein Binding</term><term>Protein Interaction Mapping</term><term>Surface Plasmon Resonance</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie d'interactions entre protéines</term><term>Humains</term><term>Immunoprécipitation</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Prolifération cellulaire</term><term>Résonance plasmonique de surface</term><term>Technique de Far-Western</term><term>Techniques de double hybride</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1alpha), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin (F-actin). In vitro and in vivo interaction was then confirmed by immuno-coprecipitation, far-Western blotting, and surface plasmon resonance. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1alpha, inhibiting protein translation and cytokinesis by blocking F-actin bundling. Proliferation of human peripheral blood lymphocytes and other human cell lines was significantly inhibited by the infection of recombinant retrovirus expressing SARS-CoV N protein.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name><name sortKey="Li, Xiaorong" sort="Li, Xiaorong" uniqKey="Li X" first="Xiaorong" last="Li">Xiaorong Li</name><name sortKey="Liu, Junli" sort="Liu, Junli" uniqKey="Liu J" first="Junli" last="Liu">Junli Liu</name><name sortKey="Liu, Xuan" sort="Liu, Xuan" uniqKey="Liu X" first="Xuan" last="Liu">Xuan Liu</name><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name><name sortKey="Wang, Qiuna" sort="Wang, Qiuna" uniqKey="Wang Q" first="Qiuna" last="Wang">Qiuna Wang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Bing" sort="Zhou, Bing" uniqKey="Zhou B" first="Bing" last="Zhou">Bing Zhou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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